ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Karyokinetic and cytokinetic irregularities were examined in cultured mesophyll protoplasts of Medicago sativa L. cv. Regen S and Nicotiana tabacum L. cv. Wisconsin 38 during the early cell divisions. Spindle anomalies, laggine chromalids during anaphase, displaced phragmoplasts during telophase and incomplete cross-walls following the completion of cytokinesis were scored in these cultures. The abnormalities, however, occurred at low frequencies when compared to those found in suspension culture-derived protoplasts of Vicia hajastana. It is suggested that abnormalities are reduced because the mesophyll protoplasts regenerate a substantial cell wall prior to the onset of the first division.An entirely different class of cytological aberration occurring in 40–50% of the cells was caused by localized protruding of the cytoplasm (budding). The nucleus was often positioned at the site of constriction of the bud. The association of budding with mitosis has led us to postulate a mechanism which may be a cause of budding and to suggest a possible way to prevent it.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1988.tb00626.x
