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Isolation and analysis of gas bubbles in the cavities of Azolla leaves (1995)

Uheda, Eiji ; Kitoh, Shunji ; Dohmaru, Takaaki ; [et al.]

Copenhagen : Munksgaard International Publishers

Physiologia plantarum 93 (1995), S. 0

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ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: A procedure has been developed for the isolation of gas bubbles from the cavities of leaves of Azolla rubra. Mature leaves of exponentially growing Azolla plants were carefully cut under water with a razor blade. The gas bubbles in the cavities were driven out by a stream of water running from a glass capillary and collected in another capillary that was filled with distilled water. This allowed the collection of a sufficient number of gas bubbles for analysis. The concentration of oxygen in the gas obtained was slightly lower than that in the external air. The diffusion of external 15N2 into the gas bubbles was low. The concentration of 15N2 in gas bubbles was only 2% of that of the external air even after incubation of Azolla plants in 15N2 in light or in darkness for 20 h.The method described here for isolation and analysis of gas bubbles should permit further studies of the properties of the gas bubbles in the cavities of Azolla leaves.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1034/j.1399-3054.1995.930101.x

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