Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
The @journal of eukaryotic microbiology
13 (1966), S. 0
ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
SYNOPSIS. The effects of the trypanocidal drug, pentamidine, on the ribosomal fraction of Crithidia oncopelti have been investigated in vivo and in vitro. Addition of the drug to ribosomes isolated from the organisms led to release of magnesium and some protein from the particles. The ribosomes were aggregated when significant release of magnesium had occurred. Loss of magnesium was dependent on density of the ribosomal suspension but not on the magnesium concentration. Spermine, propamidine and stilbamidine had a similar effect in releasing magnesium from the particles. The majority of pentamidine (80%) or stilbamidine (70%) added to suspensions of ribosomes became bound to the particles and could not readily be removed by washing. Stilbamidine was not released in the presence of adenosine triphosphate. Slight stabilisation of 80S particles was observed in the presence of pentamidine and absence of magnesium.Incubation of organisms in the presence of growth-inhibitory concentrations of pentamidine led to no significant change in the ribosomal content of the organisms, even when complete inhibition of the ability to incorporate C14-glycine into nucleic acid and protein had occurred. There was no significant decrease in the magnesium content of ribosomes isolated from organisms incubated in the presence of pentamidine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1966.tb01900.x
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