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The Methylated Purines of Tetrahymena pyriformis Transfer Ribonucleic Acid (1979)

KEEGAN, FRANK P. ; BERECH, JOHN

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 26 (1979), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: SYNOPSIS. By phenol extraction and DEAE cellulose column chromatography, tRNA was isolated from Tetrahymena pyriformis strain GL. Following acid hydrolysis of the tRNA, the methylated purine content was determined by Dowex 50 column chromatography and paper chromatography.The most abundant methylated guanine derivative was found to be N2-DMG. Also present were 1-MG, N2-MG, and 7-MG. The most abundant methylated adenine was found to be 1-MA; no 2-MA was detected. Small amounts of the N6-methyladenines were detected.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1979.tb04660.x

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