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New Methods for Cultivating, Harvesting, and Purifying Mass Cultures of the Hypotrich Ciliate Euplotes aediculatus (1982)

SCHMIDT, HELMUT J.

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 29 (1982), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: A new method is described for mass cultivation of Euplotes aediculatus, a hypotrich ciliate containing omikron-symbionts. The ciliate cultures, continuously aerated in Erlenmeyer flasks (5000 ml) with 4500 ml medium, yield densities of 2300 cells/ml which are four to five times higher than cell densities of cultures grown in unaerated Fernbach flasks. Harvesting such cultures involves the application of 25-μm mesh sieves. Cells so concentrated can be purified by using columns or special chambers in which Euplotes migrates towards the cathodes in an electric field (field strength 7 V/cm).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1982.tb02896.x

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