ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
SYNOPSIS. Rabbit antisera to trophic and resting cyst stages of C. maupasi were analyzed by absorption and cross absorption with trophic and resting cyst antigens. The trophic form gave rise to immobilizing and agglutinating antisera of high titer, whereas the cyst stage gave rise to immobilizing and agglutinating antisera of lower titer. The cyst antiserum also stimulated the formation of a gelatinous membrane around some of the immobilized organisms. Both antisera had their effect against the trophic form; the cyst stage was not visibly affected.Two antigenic components were demonstrated in the resting cyst stage, one shared in common with the trophic stage, which gave rise to an immobilizing and agglutinating antibody, and a second antigen present only in the cyst, yielding a lytic antibody. The lytic antibody was revealed only after absorption of the immobilizing antibody component from the cyst antiserum.The recombination of immobilizing and agglutinating antibodies with lytic antibody revealed a reciprocal linear relationship, i.e., the lytic activity was more pronounced as the immobilizing titer decreased. The two antibody components when combined in a ratio of approximately 3:1 (immobilizing to lytic antibody), inhibited lysis and duplicated the immobilizing and gelatinous membrane formation observed for the unabsorbed cyst antisera.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1962.tb02572.x
