ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
For a continuous process using maltotetraose (G4)-forming amylase in a membrane recycle bioreactor (MRB), the enzyme was partially purified to specific activity 21.5 IU/mg protein. The enzyme was stable enough to convert starch into G4 in batch and MRB systems. Optimum conditions in the MRB using hollow fiber with 1,000 molecular weight cut-off were: substrate concentration 1.0% (w/v), enzyme concentration 70 IU/L reactant, and residence time 83 min. Productivity at steady state under optimized conditions was 0.99 g/L/hr. Excessive residence time decreased product purity by further conversion of G4 to lower molecular weight compounds due to endo-acting activity of the enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1991.tb14631.x
