ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract A commercial type liquid culture medium for the production of Colletotrichum truncatum NRRL 13737, a pathogen of the weed hemp sesbania, was developed. The concentrations of culture ingredients: 20 g glucose 1−1, 2.5 g Tastone yeast extract 1−1, 7.5 g Pharmamedia 1−1 are optimum for batch cultures. Initial glucose concentration determined total dry weight which was largely composed of mycelia: Pharmamedia concentration influenced the yield and rate of spore production; Tastone 154 yeast extract apparently supplied a nutritional factor which also affected the yield and rate of spore production but to a lesser extent. Batch culture with the described media yielded 6.2×107 spores per ml in three days. Studies of spore recovery, drying and storage can now be conducted with a commercial type culture medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1993.tb06042.x
