Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
50 (1988), S. 0
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) followed by immunoblotting was employed to detect intracellular precursors of endo-β-1,4-glucanases (EGs) in Trichoderma reesei QM9414 under conditions of de novo induction by sophorose and de novo carbon catabolite derepression by lactose. Secretion of EGs was always preceded by intracellular accumulation of lower Mr precursors, which became processed to larger Mr forms immediately prior to their extracellular appearance. Treatment of the larger Mr forms with α-mannosidase converted them to forms with the same Mr as the smaller forms, whereas Endo H treatment was without effect. These results are consistent with a requirement of O-linked glycosylation for secretion of EGs by T. reesei.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1988.tb02943.x
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