ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The effect of changes in the intracellular concentration of α-aminoadipate on the formation of α-aminoadiphyl-cysteinyl-valine (ACV) and isopenicillin N (IPN)—two intermediates of penicillin biosynthesis—by strains of Penicillium chrysogenum has been investigated by measuring the incorporation of radioactivity from (6−14C)-α-aminoadipate into cellular 14C-ACV and 14C-IPN. No ACV or IPN were found in any strain during cultivation on glucose, but were clearly detected in all three strains during growth on lactose, displaying increased formation in strains exhibiting increased penicillin productivity and increased intracellular α-aminoadipate pools. ACV and IPN formation was affected by subjected P. chrysogenum mycelia to either general amino acid control (by addition of amitrol) or by exogenous addition of 5 mM l-lysine. In all cases, the changes observed paralled the changes in the cellular α-aminoadipate pool. These results are consistent with the α-aminoadipate pool limiting the biosysthesis of ACV and IPN and hence penicillin biosynthesis in the present strains of P. chrysogenum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1989.tb03599.x
