ISSN:
1662-9779
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Physics
Notes:
We report on the distribution of mixed self-assembled monolayers (SAMs) composed of biotinylatedand diluent alkylthiolates for streptavidin immobilization. Two thiol derivatives,11-mercapto-1-undecanol (MUOH) and 11-mercaptoundecanoic-(8-biotinylamido-3,6-dioxaoctyl)amide (MBDA), were employed for mixed SAM. These thiols formed self-assembled monolayerwithout local domain, and streptavidins were immobilized onto biotinylated gold surface withoutnonspecific binding. In order to find the optimized condition of immobilization of streptavidin, wecontrolled the mixing ratio of two kind thiols by colorimetric detection assay, and the immobilizationwas characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM), andellipsometer
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/02/23/transtech_doi~10.4028%252Fwww.scientific.net%252FSSP.121-123.495.pdf