ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The nacre layer from the pearl oyster shell is considered as a promising osteoinductivebiomaterial. Nacre contains one or more signal molecules capable of stimulating bone formation.The identity and the mode of action of these molecules on the osteoblast differentiation wereanalyzed.Water-soluble molecules from nacre were fractionated according to dialysis, solvent extraction andreversed-phase HPLC. The activity of a fraction composed of low molecular weight molecules inthe mineralization of the MC3T3-E1 extracellular matrix was investigated. Mineralization of thepreosteoblast cells was monitored according to alizarin red staining, Raman spectroscopy, scanningelectron microscopy and quantitative RT-PCR.Molecules isolated from nacre, ranging from 50 to 235 Da, induced a red alizarin staining of thepreosteoblasts extracellular matrix after 16 days of culture. Raman spectroscopy demonstrated thepresence of hydroxyapatite in samples treated with these molecules. Scanning electron microscopypictures showed at the surface of the treated cells the occurence of clusters of spherical particlesressembling to hydroxyapatite. The treatment of cells with nacre molecules accelerated expressionof collagen I and increased the mRNA expression of Runx2 and osteopontin.This study indicated that the nacre molecules efficient in bone cell differentiation are certainlydifferent from proteins, and could be useful for in vivo bone repair
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1017.pdf
