ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Grafting of autologous iliac crest and decortication approach in posterior spinal fusionsurgery has been the “gold standard”. However, the limited source of autograft has promptedextensive research into bone substitute and biological enhancement of the fusion mass. In this study,the application of stem cell therapy by tissue engineering method was investigated to enhanceposterior spinal fusion with [removed info]-tricalcium phosphate ceramics in rabbit model. Rabbit bone marrowderived mesenchymal stem cells were aspirated from trochanter region of proximal femur. Themesenchymal stem cells were grown and directed to differentiate into osteogenic cells byosteogenic supplement (ascorbic acid, [removed info]-glycerophosphate and dexamethasone) in basal medium(10% FBS in DMEM). The osteogenic cells were seeded on tricalcium phosphate ceramics for oneday (MSC group, n=6). The cell-ceramics composite was implanted onto autologous L5 and L6transverse processes with decortication approach in posterior spinal fusion. The cell free ceramicsacts as control (Control group, n=6) and iliac crest autograft as positive control (Autograft group).The spinal segments were harvested at week 7 post-operation. Manual palpation was performedwith spinal segments to assess any movement of L5-L6 vertebral joint. The stiffness of the joint wasconsidered as solid fusion. The specimens then were fixed by formalin and transferred to 70%ethanol. The BMC and volume of fusion transverse processes of L5 and L6 was measured byperipheral quantitative computed tomography.In manual palpation, 50% solid fusion was found in MSC group, 60% in autograft group butnone in control group. Moreover, the BMC of L5 and L6 transverse processes in MSC group wasgreater than autograft and control group (45%, 40% respectively, p〈0.01). The volume of transverseprocesses in MSC group was greater than autograft by 45% (p〈0.01) and control group by 26%(p〈0.05). In conclusion, the mesenchymal stem cells derived osteogenic cells augmented spinalfusion and bone mineralization
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.334-335.1201.pdf
