ISSN:
1662-9752
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The contact of a cell on the biomaterial’s surface is mediated by its adhesioncomponents. The topography of titanium surfaces influences these adhesion components ofosteoblasts, e.g. the integrins, the adapter proteins and the actin cytoskeleton.In our current experiments we were interested in why osteoblasts were strongly aligned to thegrooves of a structured pure titanium surface (grade 2). The titanium was characterized by EIS toget insights in the electro-chemically active surface. We used MG-63 human bone cells, cultured inDMEM with 10% FCS at 37°C. For protein adsorption the titanium discs were incubated for 24hwith complete medium containing soluble fibronectin at 37°C. Interestingly, only in the groovescells adhered and were aligned and this is not dependent on the gravitation. The cell adhesion seemsto depend on the protein adsorption of fibronectin which we could find to be adsorbed exclusivelyin the valleys. We speculate that there are local differences in electro-chemical characteristics ofthis structured titanium surface
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/02/14/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.517.pdf
