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Fluorescence line-narrowing spectra of Zn-cytochrome c. Temperature dependence (1987)

Koloczek, Henryk ; Fidy, Judit ; Vanderkooi, Jane M.

College Park, Md. : American Institute of Physics (AIP)

The Journal of Chemical Physics 87 (1987), S. 4388-4394

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ISSN: 1089-7690

Source: AIP Digital Archive

Topics: Physics , Chemistry and Pharmacology

Notes: Fluorescence site selection spectra of Zn-substituted cytochrome c and Zn-mesoporphyrin were measured as a function of excitation energy and temperature within 5–60 K. The emission energies determined for Zn-cytochrome c were comparable with those of Zn-mesoporphyrin and mesoporphyrin in polar glassy matrices and with resonance Raman spectra of heme proteins. In the site selection spectra of both Zn-cytochrome c and Zn-mesoporphyrin in polar organic glass an increase in the intensity of zero phonon emission lines was observed with an increase of temperature between 5 and 30 K. The irregular spectral changes were interpreted on the basis of the temperature dependence of the linewidth as well as possible hole-burning phenomena.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1063/1.452899

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