ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The MspI restriction endonuclease is a type II restriction enzyme. Unlike all other restriction enzymes with known structures, MspI recognizes the palindromic tetranucleotide sequence 5′-C/CGG and cleaves it as indicated by the `/' to produce DNA products with 5′ two-base overhangs. Owing to the nature of its cleavage pattern, it is likely that MspI would represent a new structural class of restriction endonucleases. Crystals of the dimeric MspI restriction enzyme bound to a duplex DNA molecule containing the specific recognition sequence have been obtained by vapor-diffusion techniques in the presence of polyethylene glycol as precipitant. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 50.2, b = 131.6, c = 59.3 Å, β = 109.7°. The crystals contain one dimeric complex in the asymmetric unit. A complete native data set has been collected to a resolution of 2.05 Å by cryo-crystallographic methods, with an Rmerge of 4.0%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444900012713
