ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Sep-Pak CN cartridge
;
Oxytetracycline
;
Sulphadimidine
;
Meat and eggs
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A rapid method for the simultaneous determination/identification of residual oxytetracycline (OTC) and sulphadimidine (SDD) in meats (beef, pork, chicken) and eggs by high-performance liquid chromatography (HPLC) was developed. The extraction of OTC and SDD was performed using a Sep-Pak® CN cartridge. The extracts contained OTC/SDD analytes when examined by HPLC using a LiChrospher® 100 RP-8 end-capped column and a mobile phase of acetonitrile-acetic acid-water (28:4:68, v/v/v) with a photodiode array detector. The average recoveries from spiked samples (0.1 μg g−1 and 1.0 μg g−1) were in excess of 80.2% with coefficients of variation between 1.5 and 5.0%. The limits of detection for OTC and SDD were 0.05 and 0.02 μg g−1, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467608
