ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Chlorophyllc
;
Marine phytoplankton
;
Octylsilica phases
;
Photosynthetic pigments
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The separation of mono- and divinyl chlorophyllc forms was estudied employing several octylsilica and octadecylsilica columns under isocratic conditions. In this paper we show that, using the adequate mobile phases, the monomeric C8 columns can separate the mentioned chlorophylls. A comparison between C8 and C18 columns reveals that monomeric OS phases provide always higher resolution of the MV- and DV chlorophyllc pairs. Such a result could be explained in terms of differences in column polarity. When adequate gradient profiles and injection conditions are used the separation is accomplished together with that of other chlorophylls and carotenoids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467598
