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Comparative study on determination of fumagillin in fish by normal and reversed phase chromatography (1998)

Fekete, J. ; Romvári, Zs. ; Gebefügi, I. ; [et al.]

Springer

Chromatographia 48 (1998), S. 48-52

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Normal- and reversed-phase LC ; Fumagillin in fish samples

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary Fumagillin is an antibiotic agent mostly used in the veterinary profession. A normal-phase liquid chromatographic method was developed for the determination of this drug in fish matrices. A hexane-dichloromethanedioxan-2-PrOH-acetic acid (43:43:9:5:0.1 v/v %) eluent was used on a Perkin Elmer silica gel column. For validation of the process a reversed-phase HPLC method was also developed (eluent: acetonitrile-water-bicyclohyxylamine 70:30:0.05 v/v %, with Spherisorb ODS, as stationary phase). Recovery is about 100% and the limit of detection is 5 ng g−1 for meat samples.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02467515

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