ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Indomethacin in serum
;
Fluorometric detection
;
In-line alkaline hydrolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A fluorometric method for determining indomethacin in serum by reversed-phase high performance liquid chromatography has been developed. Deproteinized serum containing indomethacin was injected directly onto a C18-bonded vinyl alcohol copolymer column with an alkaline mobile phase (pH 10.0, 35% acetonitrile in phosphate buffer), and was detected fluorometrically (Ex. 298 nm and Em. 375 nm) via postcolumn in-line alkaline hydrolysis at high temperature (140°C). The calibration curve was linear over the range of 0.1–10.0 μg/ml when injecting a volume of 10 μl of deproteinized serum. The detection limit (signal-to-noise ratio=3) for indomethacin in serum was 10 ng/ml using a 20-μl aliquot of deproteinized serum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02275910
