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Synthesis of affinity chromatography and electrophoresis matrices for the purification of bile acid transport proteins (1991)

Müllner, S. ; Möller, W. ; Ziegler, K. ; [et al.]

Springer

Chromatographia 32 (1991), S. 265-268

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ISSN: 1612-1112

Keywords: Affinity chromatography ; Affinity electrophoresis ; Bile acid transport proteins ; Bile acid derivatives

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary For the purification of the bile acid transport proteins from rat hepatocytes high affinity taurocholic acid derivatives were synthesized. One type was designed for coupling to a Sepharose matrix and the other for copolymerization with acrylamide. The resulting matrices were found to be suitable for affinity chromatography and affinity electrophoresis. In both syntheses, 3α, 12α-dihydroxy-7β-amino (tauro) cholic acid derivatives were used as the starting material, since substitution in this position had previously been shown not to alter transport affinity constant.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02276251

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