ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Wheat proteins
;
γ-gliadins
;
Effect of pH on separation
;
Effect of organic modifier on separation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Reversed-phase, high-pressure liquid chromatography has been used to separate similar protein (γ-gliadin) components from 70% ethanol extracts of endosperm flour from two different accessions of the diploid wheatTriticum monococcum L. The effect of acetonitrile as the organic eluent was compared to acetonitrile: 2-propanol (3:1) at two different pH's. Conditions for maximum resolution of the γ-gliadin components were found to be at pH 7.2 with acetonitrile: 2-propanol (3:1) as the eluent. These conditions allowed the components to be obtained in sufficient purity for further charaterization.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02319634