ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Size exclusion
;
Vesicular packing material
;
Proteins and polymers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A new vesicular packing material — prepared from plant cell clusters by purification of the intact cell wall framework —is suitable for chromatography, giving high performance at low pressure gradients. The separation is achieved by dialysis through the cell wall, which is an ultrafilter membrane with an extremely sharp size limit of separation. Almost the whole of the stationary liquid phase is located within the vesicle (empty cell) lumina. In contrast to gel filtration vesicle chromatography gives a practically ideal separation of two size groups with an extremely short fractionation range. The size limit of separation was investigated by chromatography of proteins and other polymers. Group separation of molecules of a polydisperse dextran standard preparation showed that the critical Stokes' diameter for dextran permeation into the stationary liquid phase of the vesicular packing is 5 to 6 nm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02260677
