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Solvent optimization in normal-phase liquid chromatography of selected steroids (1982)

Antle, P. E.

Springer

Chromatographia 15 (1982), S. 277-281

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ISSN: 1612-1112

Keywords: Liquid chromatography ; Solvent selectivity ; Steroids ; Amine bonded phase ; Overlapping resolution mapping

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary Resolution (Rs) is a function of three factors: efficiency, retentivity and selectivity. A procedure for optimization of mobile phase selectivity was demonstrated using a reversed-phase (RP) separation of compounds with various functionalities. In this paper, the same procedure is used with normal bonded-phase (NP) chromatography to develop a separation of structurally similar steroids. The optimization of mobile-phase selectivity provided increased problem-solving capability and decreased the analysis time.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02265677

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