ISSN:
1436-6215
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
Notes:
Summary The effect of exogenous adenosine triphosphate on the oxygen uptake of rat diaphragm and liver slices and homogenate were studied in normal rats using Warburg Manometric Technique. Five concentrations of ATP were used, namely 0.275, 0.55, 1.1, 2.75, and 5.5 mM ATP. ATP in small concentrations showed no significant changes in oxygen uptake of rat diaphragm and muscle homogenate or liver slices, but the oxygen uptake of liver homogenate was significantly increased in presence of 0.55 mM ATP while large concentrations caused depression in oxygen uptake of rat diaphragm and liver tissue whether it was sliced or homogenate. The depressant action of large concentrations of ATP on oxygen uptake by liver and muscle tissues is explained by the finding that the activity of respiration is dependent on the ratio of ATP/ADP. The stimulatory effect of the small concentration of ATP in case of liver homogenate and the absence of its effect in case of liver slices may be due to the different action of ATP with respect to adenyl cyclase in homogenate and slices. The oxygen uptake of homogenate was significantly greater than that of slices. This is presumably due to the greater excess of the intracellular respiratory mechanisms in the homogenate than in the slices. However, the recent discovery of the purinergic nerve hypothesis and the finding that exogenously applied ATP mimics the action of the nerve-released transmitter will greatly improve the understanding of the action of ATP.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02026536
