ISSN:
1615-6102
Keywords:
Dactylis glomerata L.
;
Orchardgrass
;
Sodium chloride tolerance
;
Somatic embryo
;
Tissue culture
;
Ultrastructure
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Calli were induced from leaf expiants of aDactylis glomerata L. (orchardgrass) genotype which has a high capacity for somatic embryogenesis. After 7 months culture on SH medium containing NaCl, a line was selected which was tolerant to 200 mM NaCl. When both selected and nonselected calli were maintained for 56 days on media containing 0 to 300 mM NaCl, the selected line showed significantly higher regeneration capacity than nonselected calli when placed on media containing more than 50 mM NaCl. Ultrastructural features of control somatic embryos not exposed to the salt were compared to those from nonselected and selected embryos cultured on 200 mM NaCl medium. In the presence of NaCl there were changes in the appearance of cell walls and mitochondria, accumulation of lipids and a higher degree of vacuolation in cells of nonselected embryos compared to control and selected embryos.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01378792
