ISSN:
1615-6102
Keywords:
Electrofusion
;
Flow cytometry
;
Heterokaryon selection
;
Plant protoplasts
;
Somatic hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Protoplasts from a range of tissue sources for 21 species within the plant familiesCompositae, Cruciferae, Leguminosae, andSolanaceae were fused electrically to produce heterokaryons. Protoplasts were labelled by application of green fluorescein fluorescence to one parental protoplast population, and the red fluorescence of either naturally occurring chlorophyll or of rhodamine exogenously applied to the other parental type. Heterokaryon populations were identified, characterized and isolated using an EPICS 541 flow cytometer. Following culture, sorted heterokaryons from 11 of the 14 combinations investigated underwent division; in 5 of these combinations the heterokaryons developed into cell colonies, 4 produced callus and 2 gave rise to somatic hybrid shoots.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01349533
