ISSN:
1615-6102
Keywords:
Acid phosphatase
;
Electron microscopy
;
Mammary glands
;
Subcellular fractions
;
Substrate specificity
;
Sulfhydryl agents
;
Tartrate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Localization of acid phosphatase in mammary glands of lactating rats was studied by both biochemical and cytochemical methods. Cytochemically, acid phosphatase activity was detected by using lead citrate as the capture agent for the inorganic phosphate released from p-nitrophenyl phosphate. The activity was predominantly localized in the lumina of the endomembrane system and in the milk that had been secreted into the alveolar lumen. Biochemically, acid phosphatase was present in all the subcellular fractions with higher activities in the membrane-associated fractions. The localization of tartrate-resistant acid phosphatases within the endomembrane system of fully lactating rat mammary tissue suggests a possible role for these enzymes in milk secretory processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01353999
