ISSN:
1615-6102
Keywords:
Vacuolar ATPase
;
Plasma membrane
;
Endomembrane
;
Zucchini
;
Brain
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Polyclonal antibodies raised against the tonoplast and plasma membrane H+-ATPases of corn were used to probe Western blots lof clathrin-coated vesicles purified from zucchini hypocotyls and bovine brain. Antibodies to two tonoplast H+-ATPase subunits recognized their respective polypeptides in purified zucchini coated vesicle preparations. Antibody raised against the plasma membrane H+-ATPase failed to cross-react with zucchini coated vesicles, even though it recognized a 100 kDa polypeptide in purified zucchini plasma membrane preparations. These results suggest that zucchini coated vesicles contain a vacuolar-type rather than a plasma membrane-type H+-ATPase. Antibody to the corn 70 kDa subunit also cross-reacted with a 70 kDa band in bovine brain coated vesicle preparations. Surprisingly, the corn plasma membrane H+-ATPase antibody recognized a 100 kDa polypeptide in the bovine brain coated vesicle preparation. However, at least half of the 100 kDa antigen could be dissociated from the membrane by treatment with 2.0 M urea, indicating that it may represent one of the 100–110 kDa coat assembly proteins. A comparable protein appears to be absent from zucchini coated vesicles.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01322472
