ISSN:
1615-6110
Keywords:
Solanaceae
;
Solanum
;
AFLP markers
;
DNA fingerprinting
;
phylogeny
;
polyploids
;
potato
;
tomato
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Using the AFLP technique highly informative DNA fingerprints were generated from 19 taxa ofSolanum sect.Petota (potatoes) and three taxa ofSolanum sect.Lycopersicum (tomatoes). Both phenetic and cladistic analyses were conducted from the individual genotypic level to the species level. An AFLP fingerprint, using a combination of suitable AFLP primers, generated 12 to 71 scorable fragments per genotype which was sufficient for taxonomic interpretation. The classifications based on the molecular markers were generally in agreement with current taxonomic opinions. Unexpectedly,S. microdontum was associated with ser.Megistacroloba rather than with ser.Tuberosa, andS. demissum (ser.Demissa) and species of ser.Acaulia appeared closely affiliated. AFLP is an efficient and reliable technique to generate biosystematic data and therefore a promising tool for evolutionary studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00984729
