ISSN:
1615-6110
Keywords:
Angiosperms
;
Asteraceae
;
Brachycome dichromosomatica
;
Suspension cultures
;
karyotype stability and mutation
;
chromatin ultrastructure
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A long-term suspension culture ofBrachycome dichromosomatica (2n = 4) was induced from a cotyledon-derived callus. Subcultures were obtained every week up to three years. The bulk of the cultures displayed a stable diploid karyotype, while one cell line evolved with 2n = 5 chromosomes in the 86th reinoculation. No further chromosomal change occurred also in that cell line. It is assumed that the fifth chromosome is the expression of a trisomy 2. The chromatin ultrastructure was of the species-specific chromomeric type in the wild-type line, while the trisomic line displayed more condensed chromatin, what probably indicates a rather inactive state of the extra-chromosome.Brachycome dichromosomatica is suggested to represent an ideal species to follow-up karyotype stability and/or variation in cell culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00936339
