ISSN:
1435-8107
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The plant growth regulator (−)-jasmonic acid (JA) and its stereoisomer (+)-7-iso-jasmonic acid (7-iso-JA) have been isolated from young fruits ofVicia faba L. and identified by TLC, GC, GC-MS, and chemical transformation. Different isolation procedures gave different ratios of JA/7-iso-JA because of partial isomerization of (+)-7-iso-JA to (−)-JA. Optimal conditions, excluding artificial isomerization, were checked by the addition of [U-14C]-7-iso-JA. The naturally occurring isomer ratio was determined to be 65% (−)-JA: 35% (+)-7-iso-JA in immature broad bean fruits. The biological activities of both isomers of JA have been studied using several bioassays. Growth of wheat and GA3-stimulated dwarf rice seedlings is inhibited more effectively by (+)-7-iso-JA than by (−)-JA. In growing seedlings of barley and oat, both isomers caused a senescence-like bleaching effect characterized by chlorophyll and carotenoid decrease. The free acids are more active than their methyl esters in intact barley plants in contrast to results obtained in leaf segment tests. Highest activity was obtained with (+)-7-iso-JA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02025960
