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  • 1
    Electronic Resource
    Electronic Resource
    PCR amplification of 16S rDNA from lyophilized cell cultures facilitates studies in molecular systematics (1990)
    Springer
    Current microbiology 21 (1990), S. 325-327 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The sequence of the major portion of aBacillus cycloheptanicus strain SCHT 16S rRNA gene is reported. This sequence suggests thatB. cycloheptanicus is genetically quite distinct from traditionalBacillus strains (e.g.,B. subtilis) and may be properly regarded as belonging to a different genus. The sequence was determined from DNA that was produced by direct amplification of ribosomal DNA from a lyophilized cell pellet with straightforward polymerase chain reaction (PCR) procedures. By obviating the need to revive cell cultures from the lyophile pellet, this approach facilitates rapid 16S rDNA sequencing and thereby advances studies in molecular systematics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02092099
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