ISSN:
1476-5535
Keywords:
Keywords: diffusion; BSA; alginate; wavelets
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A microscopic technique has been developed to obtain the protein profiles inside calcium alginate gel. To do this, the diffusion of BSA, previously marked with FITC, inside calcium alginate beads was observed using confocal laser microscopy, thus obtaining the spatio-temporal evolution of the protein concentration. The technique, however, presents certain limitations and zones where it is impossible to obtain experimental data. Wavelets analysis, commonly used in signal processing and statistics, was employed to reconstruct and subsequently analyse the experimental results. Once the diffusion model was defined, the substrate profiles obtained were used to calculate a diffusivity value for BSA in alginate gel.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/sj.jim.2900703
