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Mammalian species identification by interspersed repeat PCR fingerprinting (1998)

Buntjer, J B ; Lenstra, J A

Springer

Journal of industrial microbiology and biotechnology 21 (1998), S. 121-127

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ISSN: 1476-5535

Keywords: Keywords: species identification; animal tissues; PCR; DNA fingerprinting

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Most DNA methods for species identification of animal tissues test the presence/absence of one species per assay, requiring several tests for a complete analysis and prior knowledge of the species that are potentially present in the sample. Here we demonstrate that PCR with fluorescently labeled MIR (mammalian-wide interspersed repeat) primers generate fingerprints that are suitable for rapid identification of known and unknown species on an automatic sequencing apparatus and with computer-assisted data processing. The method allows the analysis of processed meat samples and offers a convenient alternative to sequencing of mitochondrial DNA.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/sj.jim.2900540

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