ISSN:
1432-2285
Keywords:
Mycorrhizae
;
Paxillus
;
Pinus
;
Root exudate
;
Roots
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Seedlings ofPinus resinosa Ait. in test tubes were inoculated with the ectomycorrhizal fungusPaxillus involutus Fr. or with discs of sterile modified Melin-Norkrans (MMN) medium.Paxillus involutus was also inoculated to control tubes in the absence ofPinus resinosa seedlings. In vivo labelling of proteins inPinus resinosa roots and inPaxillus involutus mycelium was carried out using35Sl-methionine 1, 2, 3, 4, 5 and 7 days after inoculation. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) of the protein extracts from the four treatments and autoradiography demonstrated that the presence of root exudates altered protein synthesis inPaxillus involutus as three major bands disappeared whenPaxillus involutus was exposed to root exudates. Protein synthesis inPinus resinosa was also altered whenPaxillus involutus was introduced into the tubes, since at least two bands were more intense when seedlings were inoculated withPaxillus involutus, as compared to control roots. No difference was observed in the growth and the label incorporation ofPaxillus involutus growing with or without root exudates. Ectomycorrhizal roots were not formed during this experiment. Gene regulation in this ectomycorrhizal association occurs, therefore, prior to the formation of ectomycorrhizal roots.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01021069
