ISSN:
1432-0878
Keywords:
Blood platelets
;
Oxidized LDL
;
Cytochalasin D
;
Reflection contrast microscopy
;
Image analysis
;
Electron microscopy
;
Human
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The adhesion of human blood platelets is studied with an in vitro model using reflection contrast microscopy and an image analysis system. The adhesive feature is promoted by oxidatively modified low density lipoprotein, which also induces functional morphological changes of platelets. However, when washed platelets are pretreated with 0.05 mM cytochalasin D, oxidized low density lipoprotein (100 μg/ml) causes a slower increase of the adhesion area (11.6 μm2/min) compared to untreated platelets (15.7 μm2/min) or platelets treated by oxidized low density lipoprotein alone (20.5 μm2/min, P〈0.01). These results are supported by light transmission analysis and by transmission electron microscopy. Our experiments suggest that cytochalasin D inhibits the change of platelets in shape induced by oxidized low density lipoprotein, hinders the adhesion, but does not prevent the adhesion entirely.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00304523
