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Altered formation of dye-induced lysosomes in mouse fibroblasts due to growth in “deficient” media (1974)

Snipes, R. L. ; Wittekind, D. ; Kretschmer, V.

Springer

Cell & tissue research 147 (1974), S. 219-235

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ISSN: 1432-0878

Keywords: Lysosomes ; Mouse fibroblasts ; Dye treatment ; Cell culture ; Electron microscopy

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Mouse fibroblasts were grown in a ‘deficient’ medium consisting solely of Hank's salt solution. Treatment of cells after 48 hours in deficient medium with the cationic dye, mepacrine, resulted in an altered formation of lysosomes. Cells grown in full medium form dense, multi-vesiculated lysosomes in response to mepacrine. Cells grown in deficient medium form large electron lucent vacuoles containing peripherally located dense globules. The significance of the observations in relation to the deficiency of the medium is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00582797

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