ISSN:
1617-4623
Keywords:
Key words Chitin synthase
;
Multigene family
;
Cell wall
;
Conidia
;
Aspergillus nidulans
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We previously isolated three chitin synthase genes (chsA, chsB, and chsC) from Aspergillus nidulans. In the present work, we describe the isolation and characterization of another chitin synthase gene, named chsD, from A. nidulans. Its deduced amino acid sequence shows 56.7% and 55.9% amino acid identity, respectively, with Cal1 of Saccharomyces cerevisiae and Chs3 of Candida albicans. Disruption of chsD caused no defect in cell growth or morphology during the asexual cycle and caused no decrease in chitin content in hyphae. However, double disruption of chsA and chsD caused a remarkable decrease in the efficiency of conidia formation, while double disruption of chsC and chsD caused no defect. Thus it appears that chsA and chsD serve redundant functions in conidia formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050353
