ISSN:
1617-4623
Keywords:
Key words Histone H3
;
Introns
;
Multigene families
;
Nucleotide sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Several variants of the replacement histone H3 genes from soybean, barley and wheat have been cloned and sequenced. Analysis of segregating populations in barley and soybean, as well as analysis of clones isolated from a soybean genomic library, suggested that these genes are dispersed throughout the genome. Several genes contain introns located in similar positions, but of different lengths and sequence. Comparison of mRNA levels in different tissues revealed that the intron-containing and intronless genes have different expression patterns. The distribution of the introns in the histone H3 genes across several plant species suggests that some of the introns might have been lost during the evolution of the gene family. Sequence divergence among introns and gene-flanking sequences in cloned gene variants allowed us to use them as specific probes for localizing individual gene copies and analyzing the genomic distribution of these variants across a range of genotypes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02174172
