ISSN:
1617-4623
Keywords:
Frankia
;
Nod
;
Nodule
;
Complementation
;
Mutant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Clones from aFrankia At4 gene bank were pooled into groups and mass conjugated into anodD mutant ofRhizobium leguminosarum bv.viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containingFrankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement anodD mutant ofR. leguminosarum bv.viciae. Thus pAt2GX contains aFrankia gene that is functionally equivalent tonodD ofR. leguminosarum bv.viciae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00587593