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Localization of nif genes on a large plasmid in Frankia sp. strain ULQ0132105009 (1986)

Simonet, Pascal ; Haurat, Jacqueline ; Normand, Philippe ; [et al.]

Springer

Molecular genetics and genomics 204 (1986), S. 492-495

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ISSN: 1617-4623

Keywords: Frankia ; Nif genes ; Nitrogen fixation ; Plasmid ; Actinorhizal symbiosis

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary We report that in DNA from one Frankia sp. strain at least some of the nif genes are located on a large indigenous plasmid of 190 kb. Using the cloned nitrogenase structural genes from Klebsiella pneumoniae as hybridization probes, homology was detected with a 5.6 kb EcoRI fragment from the Frankia sp. plasmid. This 5.6 kb fragment was cloned; used as a probe it hybridized to the nif K, D and H genes from Rhizobium meliloti and Klebsiella pneumoniae. The nif probes also hybridized on total DNA blots to a 10 kb EcoRI fragment that is not present on plasmid DNA, suggesting that the nif genes could be located on more than one replicon.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00331030

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