ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Four chlorate-resistant cell lines were selected from cells derived from mesophyll tissue of a dihaploid plant of Nicotiana tabacum L. Two of those cell lines, clr0 and clr19, have a high level of resistance to chlorate, cannot assimilate nitrate and lack NADH-nitrate reductase activity. The other two cell lines, clr15 and clr30, have a lower level of resistance to chlorate, can assimilate nitrate and have an altered level and regulation of NADH-nitrate reductase activity. NADH-nitrate reductase activity was partially restored in mixed extracts of clr0 and clr19 cells. Both clr0 and clr19 cells take up nitrate from the medium. Cells of the clr0 line but not those of the clr19 line lack xanthine oxidase activity. Plants regenerated from each of the cell lines, as well as secondary callus cultures initiated from those plants, exhibit the same phenotype as the primary cell lines with respect to chlorate resistance and nitrate utilization.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00325907
