ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Diamond's trypticase-yeast extract-serum-gastric mucin (TYSGM-9) medium was studied for its suitability to support the xenic growth of the oral protozoanEntamoeba gingivalis. Amoebic growth was found to be best when the inoculum for transfer was 0.1 ml, the incubation temperature was 35°C, and the interval between transfers was 48 h. These parameters were also useful for controlling the growth in xenic stock cultures, In addition, bacterial growth in xenic stock cultures, which had a direct effect on the activity ofE. gingivalis trophozoites, was kept to a minimum by addition of the antibiotics piperacillin, erythromycin, neomycin, and penicillin. Varying substitutions and selected supplementations of TYSGM-9 medium led to the development of an improved medium forE. gingivalis. Supplements most beneficial for the growth ofE. gingivalis trophozoites were ascorbic acid, ferric ammonium citrate, and special NCTC 107 vitamin mixture. As compared with TYSGM-9 medium (6–10E. gingivalis trophozoites observed per field), the newE. gingivalis medium supported excellent growth (16–20E. gingivalis trophozoites observed per field during optimal growth) of the oral protozoan. The medium is suitable for clinical isolation ofE. gingivalis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00931080
