ISSN:
1432-234X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The distribution of GYIRFamide immunoreactivity in the nervous system of Macrostomum hystricinum marinum has been demonstrated by an indirect fluorescence technique in conjunction with confocal scanning laser microscopy (CSLM). Immunostaining was extensive in both the central (CNS) and peripheral (PNS) nervous systems, revealing detailed information on the microanatomy of the peptidergic nervous system of this free-living plathelminth. In the CNS, immunoreactive nerve cell bodies and nerve fibres occurred in the brain and along two pairs of longitudinal nerve cords: the main nerve cords and the ventral nerve cords. In the PNS, immunostaining was prevalent in nerve cells and fibres innervating the pharynx and the gut. The employed antibody is directed against a recently characterised FMRFamide-related peptide (FaRP), GYIRFamide, isolated from two species of the Tricladida, Dugesia tigrina and Bdelloura candida. Phylogenetically, GYIRFamide represents the most ancient neuropeptide thus far identified within the Bilateria
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004350050023
