ISSN:
1432-2048
Keywords:
Chlamydomonas
;
Fertilization
;
Gamete fusion
;
Inositol 1,4,5-trisphospate
;
Plant cell-signalling
;
Polyphosphoinositides
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Within the first 2 h of sexual reproduction, gametes of the green alga Chlamydomonas eugametos agglutinate, fuse via their mating structures, de-agglutinate and swim off as vis-à-vis pairs. During this period, increases in intracellular inositol 1,4,5-trisphosphate levels and changes in polyphosphoinositide synthesis were associated with cell fusion. The protein-kinase-C inhibitor staurosporine (0.1–0.2 μM) inhibited the de-agglutination of pairs and therefore prevented them swimming away, while earlier stages of mating such as agglutination or cell fusion were unaffected. The results suggest that inositol 1,4,5-trisphosphate and diacylglycerol are fertilization signals in C. eugametos. The idea that they could also be fertilization signals in higher plants is discussed in relation to in vitro embryogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00199761
