ISSN:
1432-2048
Keywords:
Heterokaryon
;
Monoclonal antibody
;
Nicotiana (somatic hybridization)
;
Plasma membrane
;
Protoplast fusion
;
Somatic hybridization (selection tool)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Monoclonal antibodies generated by immunization with a plasma-membrane preparation from suspension-cultured cells of Nicotiana glutinosa L. were used in combination with fluoresceinor rhodamine-labeled goat anti-mouse immunoglobulins to identify heterokaryons in protoplast fusion procedures. Antibody labeling did not inhibit callus formation nor plantlet regeneration. The antibodies are non-invasive and surface labeling provides clear optical discrimination of true heterokaryons from unfused aggregates as well as from parental protoplasts and homokaryons. Labeling is stable throughout fusion and hence by pre-labeling parental protoplast populations the strategy is both versatile and of general applicability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392380
