ISSN:
1432-2048
Keywords:
β-Oxidation (enzyme location)
;
Mitochondrion
;
Microbody
;
Pisum (β-oxidation)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract β-Oxidation enzymes were detected both in the mitochondria and microbodies of pea cotyledons. Intact mitochondria did not show β-oxidation enzyme activity but in ruptured mitochondria this activity was high. It is apparent that the mitochondrial membrane barrier prevents rapid access of acyl-CoA substrates to matrix β-oxidation sites. Removal of the membrane barrier permits rapid access of acyl-CoAs and these enzyme activities may then be measured.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446368