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β-Glucan synthetase activity in Golgi vesicles ofPetunia hybrida (1977)

Helsper, J. P. F. G. ; Veerkamp, J. H. ; Sassen, M. M. A.

Springer

Planta 133 (1977), S. 303-308

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ISSN: 1432-2048

Keywords: β-Glucan synthetase ; Golgi vesicles ; Petunia hybrida ; Pollen tubes

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract β-Glucan synthetase activity has been demonstrated in a Golgi vesicle fraction isolated from pollen tubes ofPetunia hybrida. Thisβ-glucan synthetase activity differs from that of most other higher plants in its inability to incorporate [14C]glucose from GDP-[14C]glucose. UDP-[14C]glucose, however, is an appropriate glucose donor for this enzyme. The optimum conditions for thisβ-glucan synthetase activity are: 1 mg Golgi vesicle protein/ml reaction mixture; pH=±8 and a temperature of 25°C. The newly synthesized alkali-insoluble glucan containsβ-1,3- as well as β-1,4-glucosidic linkages.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00380693

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