ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Chironomus tentans salivary glands were incubated in vitro with 3H-leucine for three hours. After fixation of the glands and microdissection, the labeled proteins from chromosomes, nucleoli and nuclear sap were analyzed by SDS-polyacrylamide gel electrophoresis. The nucleoli display a very distinctive profile with many well resolved low molecular weight bands. The chromosomes and nuclear sap may share common proteins since similarities are found in their profiles. Both profiles are specific, however. A highly labeled protein at about 65,000 D is the main characteristic of the chromosomes. Some of the major species of the nuclear sap, at 165,000, 135,000 and 50,000 D, are very minor components of the chromosomes. The nuclear sap was further microdissected into inner and outer zones. The outer zone, which also includes the nuclear envelope, shows a 210,000 band which is absent from the inner zone.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00293420
